Baggage handlers at Stansted Airport in Essex have started a four-day strike to coincide with the Jubilee weekend.
Swissport workers walked out at about 05:30 and are not scheduled to return until 05:29 BST on Wednesday in a dispute over working patterns.
Talks to avert the action broke down on Friday. Workers also staged walk-outs in the dispute last month.
Richard Prince, general manager of Swissport Stansted, said passengers should not be disrupted.
The firm, which handles flights for Ryanair, Thomas Cook, Thomson, Atlasjet and BelleAir, said contingency plans had been made, including bringing in staff from its other bases.